Sulfated exopolysaccharide production and nutrient removal by the marine diatom Phaeodactylumtricornutumgrowing on palm oil mill effluent

Muhamad Maulana Azimatun Nur & Manasveni Kilnagar Swaminathan & P. Boelen & A. G. J. Buma

Palm oil mill effluent (POME) is the major source of environmental hazard in palm oil industry. Yet, due to its high nutrient
content, POME may offer opportunities for the growth of algae as a source of value-added compounds such as sulfated
extracellular polysaccharide (sEPS) while simultaneously removing valuable nutrients such as phosphate. The aim of this paper
was to evaluate growth, total sEPS production, and nutrient removal by the diatom Phaeodactylum tricornutum grown on POME
under a range of experimental conditions (temperature, salinity, supplementation of extra nutrients). Phaeodactylum tricornutum
was found to grow well on a range of POME concentrations, with 30% POME as optimum concentration. Nitrate and urea
addition enhanced both growth rate and final biomass, whereas phosphate significantly stimulated growth only at low temperature.
Box-Behnken response surface methodology revealed that interactions between temperature and salinity, and between
temperature and urea influenced sEPS production. The highest total sEPS (140 mg L−1) concentration was recorded at 25 °C,
2.6% salinity, and 100 mg L−1 urea addition. Our study shows that POME wastewater, supplemented with urea at relatively high
temperatures, can be considered as a potential medium for P. tricornutum to replace commercial nutrients while producing high
amounts of sEPS and removing almost 90% of phosphorous from the wastewater.

Keywords POME wastewater . Temperature . Extracellular polysaccharide . Phaeodactylum tricornutum . Box-Behnken . sEPS

Leave a Comment

Your email address will not be published. Required fields are marked *